We have investigated the superconducting (SC) gap on hole Fermi surfaces (FSs) of optimally substituted Ba(Fe0.65Ru0.35)2As2 by angle-resolved photoemission spectroscopy (APRES) using bulk-sensitive 7 eV laser and synchrotron radiation. It was found that, whereas the gap is isotropic in the kx − ky plane, the gap magnitudes of two resolved hole FSs show similar kz dependences and decrease as kz approaches ∼ 2π/c (i.e., around the Z point), unlike the other Fe-based superconductors reported so far, where the SC gap of only one hole FS shows a strong kz dependence. This unique gap structure can be understood in the scenario that the dz2 orbital character is mixed into both hole FSs due to finite spin-orbit coupling (SOC) and is reproduced by calculation within the random phase approximation including the SOC.